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Fig. 1 | Molecular Cancer

Fig. 1

From: Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells

Fig. 1

Cleavage of Notch1 in mammary cancer stem-like cells is dependent on CCR7. a-b Primary PyMT-Ccr7 WT and Ccr7 −/− mammary tumor cells were analyzed by multi-color flow cytometry for Notch1 expression within the stem cell-enriched population CD24+CD29hi. Top = representative flow cytometry plots, bottom = quantification. a Notch1 extracellular domain (ECD) cell surface expression in the PyMT-Ccr7 WT and Ccr7 −/− mammary stem cell-like population. b Notch1 intracellular domain (ICD) levels in the PyMT-Ccr7 WT and Ccr7 −/− mammary stem cell-like population. Bulk tumor cells were first labelled for extracellular CD24 and CD29 before permeabilization and staining for Notch1 ICD. a-b n = 4 mice/genotype. FMO = fluorescence minus one, MFI = mean fluorescence intensity. c Notch1 ICD levels in PyMT-Ccr7 WT secondary mammospheres, stimulated with CCL19 for varying lengths of time as indicated before lysis for Western analysis. n = 6 mice/experiment

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