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Fig. 3 | Molecular Cancer

Fig. 3

From: Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells

Fig. 3

Notch promotes CCR7-mediated stemness. a Level of cyclic AMP (cAMP) in PyMT-Ccr7 WT mammospheres with and without CCL19 stimulation and Notch inhibition, determined by an AlphaScreen cAMP assay. Cells were treated with CCL19, CCL21 and RO4029097 for 30 min before analysis. b PyMT-Ccr7 WT secondary mammospheres were tested for phosphorylation of ERK as a read-out for CCR7 activation with and without ligand stimulation and Notch inhibition. Cells were treated with CCL19 and RO4029097 for 15 min before lysis for Western analysis. c Secondary mammosphere-forming efficiency (number of spheres/cells seeded) of PyMT-Ccr7 WT primary mammary tumor cells cultured with and without chemokines CCL19 and CCL21, and RO4029097. ac n = 6 mice/experiment

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