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Fig. 3 | Molecular Cancer

Fig. 3

From: Targeting CD133 improves chemotherapeutic efficacy of recurrent pediatric pilocytic astrocytoma following prolonged chemotherapy

Fig. 3

MDR1 is overexpressed and plays a critical role in drug resistant pediatric PA Res186 and Res199 cells. a Light micrographs (bright field panels) showing morphology and confocal micrographs (fluorescence panels) showing MDR1 expression with immunofluorescence in drug sensitive (WT), and DOX-R, VIN-R and VCR-R pediatric PA Res186 and Res199 cells. Nuclei are labeled with DAPI (blue). b and c RT-PCR and western blots show ABCB1 (b) and its encoded protein MDR1 (c) in WT and drug resistant Res186 and Res199 cells. d MTS assay showing decreased cell viability of DOX-R, VIN-R, and VCR-R Res186 and Res199 cells in response to 0.1 μg/ml of DOX, VIN or VCR, without (control) or with 10 μM verapamil for 72 h. e and f Nuclei labeled with DAPI in DOX-R, VIN-R, VCR-R Res186 and Res199 cells treated with 10 μM verapamil for 48 h, or non-verapamil treated controls; fragmentation is indicated by yellow arrows (e), and quantitated (f). (For b, d and f, each column represents the mean ± s.d. of a minimum of three independent experiments done in triplicate; * p < 0.05, ** p < 0.01)

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