Fig. 4

CD133 promotes MDR1 expression through PI3K/AKT/NF-κB signaling in pediatric PA Res186 and Res199 cells and decreases cell chemosensitivity. a CD133 and MDR1 co-localize in WT, DOX-R, VIN-R and VCR-R Res186 and Res199 cells (white arrows). b Western blot results for CD133, total Akt, p-Akt (S473 and T308), NF-κB/p65, p-NF-κB/p65 and MDR1 in WT and Dox-R, VIN-R, VCR-R Res186 and Res199 cells. c and d Real-time PCR for ABCB1 and western blot results for CD133, total Akt, p-Akt (S473 and T308), NF-κB/p65, p-NF-κB/p65 and MDR1 in WT Res186 and Res199 cells transfected with pCMV6-Myc-DDK (pCNV6) and pCMV6-CD133-Myc-DDK (pCMVCD133). e and f Nuclear fragmentation and cell viability of WT Res186 and Res199 cells transfected with pCMV6-Myc-DDK (pCNV6) and pCMV6-CD133-Myc-DDK (pCMV6-CD133) in response to 0.01 μg/ml DOX, VIN and VCR treatments for 48 h. (For c, e and f, each column represents the mean ± s.d. of a minimum of three independent experiments done in triplicate; * p < 0.01, ** p < 0.01)