Fig. 3

Dclk1⁺ cells isolated from the IECs of Apc ^Min/+ mice display enhanced self-renewal ability and enriched tumor stem cell markers and pro-survival signaling. a Enteroids formation of isolated Dclk1⁺ cells (100 cell per well) from the small intestines of WT and Apc ^Min/+ mice. b Stacked bar and line graph represent the quantification of the number of enteroids formed and spheroid volume from the Dclk1⁺ cells isolated from WT and Apc ^Min/+ mice. c & d mRNA and protein expression of Dclk1, Bmi1, Lgr5, and Msi1 in the isolated IECs of Apc ^Min/+ mice compared with WT mice. e & f mRNA and protein expression of pro-survival signaling and their downstream targets in the isolated IECs of Apc ^Min/+ mice compared with WT mice. All quantitative data are expressed as means ± SD of a minimum of three independent experiments. P values of <0.05 = *, <0.01 = **, and 0.001 = *** were considered statistically significant