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Fig. 3 | Molecular Cancer

Fig. 3

From: Dclk1, a tumor stem cell marker, regulates pro-survival signaling and self-renewal of intestinal tumor cells

Fig. 3

Dclk1+ cells isolated from the IECs of Apc Min/+ mice display enhanced self-renewal ability and enriched tumor stem cell markers and pro-survival signaling. a Enteroids formation of isolated Dclk1+ cells (100 cell per well) from the small intestines of WT and Apc Min/+ mice. b Stacked bar and line graph represent the quantification of the number of enteroids formed and spheroid volume from the Dclk1+ cells isolated from WT and Apc Min/+ mice. c & d mRNA and protein expression of Dclk1, Bmi1, Lgr5, and Msi1 in the isolated IECs of Apc Min/+ mice compared with WT mice. e & f mRNA and protein expression of pro-survival signaling and their downstream targets in the isolated IECs of Apc Min/+ mice compared with WT mice. All quantitative data are expressed as means ± SD of a minimum of three independent experiments. P values of <0.05 = *, <0.01 = **, and 0.001 = *** were considered statistically significant

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