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Fig. 4 | Molecular Cancer

Fig. 4

From: Metalloprotease-disintegrin ADAM12 actively promotes the stem cell-like phenotype in claudin-low breast cancer

Fig. 4

ADAM12 knockdown reduces tumor formation in NOD-SCID mice after orthotopic cell transplantation. a Tumor incidence in the limiting dilution transplantation assay. SUM159PT_shADAM12 and SUM159PT_shControl cells were transplanted in the fourth mammary fat pad of NOD-SCID mice in limiting dilutions (10,000, 1,000, or 100 cells per injection site). Prior to transplantation, cells were pre-treated with doxycycline (Dox; 1 μg/ml) for 4 days, and doxycycline (2 g/kg) was then continually administered in the diet. The presence of palpable tumors was determined 3 weeks after cell transplantation. The frequency of breast tumor initiating cells (BTICs) and P-values for the statistical significance between groups were calculated using extreme limiting dilution analysis (ELDA, http://bioinf.wehi.edu.au/software/elda/; ref. [44]). b, d Representative tumors 38 days after injection of 105 SUM159PT_shADAM12 cells (b) or SUM159PT_shControl cells (d). c, e Tumor growth was monitored for 0-38 days. Nonlinear regression curves were fitted using GraphPad software

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