Fig. 7

Inhibition of NF-κB by PDTC attenuates RASSF8 knockdown-induced cell growth and invasion. a NF-κB transcriptional activity was determined by luciferase assay in cells treated with PDTC or control. b–c SGC-7901 and MGC-803 cells transfected with RASSF8 siRNA or control were treated with or without PDTC, and then subjected to colony formation assay (b) and cell invasion assay (c). *P < 0.05. d Schematic representation of the regulatory mechanism of miR-224 in cell growth, migration and invasion in gastric cancer cells under hypoxia. Hypoxia induced HIF-1α expression, which can upregulated miR-224 at the transcriptional level. MiR-224 enhanced cell growth, migration and invasion through suppressing RASSF8, which was a direct target of miR-224. RASSF8 can modulate NF-κB transcriptional activity, which can reversely influence HIF-1α expression