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Fig. 4 | Molecular Cancer

Fig. 4

From: HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells

Fig. 4

HOXC8 expression is downregulated in TN and HER2-E CSC by epigenetic silencing. a HOXC8 genomic copy number in CSC sorted as CD44+/CD24low/- cell population as determined by SYBR® qRT-PCR. Results are presented as fold difference relative to HMEC used as calibrator (n = 4). Dotted lines represent the range of normal copy number 1 ± 95% confidence interval. b Expression of miR196-a and miR196-b in CSC sorted as CD44+/CD24low/- cell population as determined by SYBR® qRT-PCR. Results are presented as relative fold expression relative to RNU6B and HMEC used as calibrator (n = 3). Relative fold expression levels were analysed by One-way ANOVA followed by Bonferroni’s multiple comparisons test. ***P < 0.001. c DNA methylation profile of HOXC8 promoter CpG Island. DNA methylation was analysed by direct PCR bisulfite sequencing. Black circles indicated methylated CG dinucleotides, white circles indicate unmethylated CG dinucleotides, white/black semicircles indicate partially methyalted CG dinucleotides. d DNA methylation profile of HOXC8 promoter of TCGA clinical samples profiled by Illumina Infinium HumanMethylation 450 BeadChip array (n = 872). Left panel shows DNA methylation normalised proportions in normal and tumour samples as visualised in UCSC Cancer Browser (blue colour represents lower methylation, red colour represents higher methylation). Right panel shows DNA methylation levels in tumour samples (red) compared to normal (green) as visualised by MethHC browser. Statistical analysis was performed by Student’s t-test. **P < 0.01

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