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Fig. 5 | Molecular Cancer

Fig. 5

From: Doxycycline attenuates breast cancer related inflammation by decreasing plasma lysophosphatidate concentrations and inhibiting NF-κB activation

Fig. 5

Doxycycline (Dox) inhibited LPA and TNFα-induced translocation of NF-κB p65 to the nucleus in breast cancer cells. Cells were serum starved for 14 h in DMEM/0.1% BSA and then followed stimulation with 5 μM LPA or 20 ng/ml TNFα. For Dox-treated cells, 5 μg/ml of Dox was included during serum starvation and stimulation. a. Time course of NF-κB translocation to nucleus induced by LPA in 4T1 cells and the effect of Dox. b. Time course of NF-κB translocation to nucleus induced by TNFα in 4T1 cells and the effect of Dox. c. Time course of NF-κB translocation to nucleus induced by TNFα in MDA-MB-231 cells and the effect of Dox. d. The effect of Dox on the time course of phospho-IκBα and total IκBα by TNFα in MDA-MB-231 cells. * p < 0.05 relative to control. Results are means ± SEM from three independent experiments. Results were analyzed by ANOVA with an SNK posthoc test

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