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Fig. 5 | Molecular Cancer

Fig. 5

From: Targeting MUC1-C inhibits the AKT-S6K1-elF4A pathway regulating TIGAR translation in colorectal cancer

Fig. 5

GO-203 inhibits cell proliferation and regress tumor growth. a SW480 (Left), LoVo (Center) and COLO-205 (Right) cells were left untreated, or were treated with 5 μM GO-203 and CP-2 each day for the indicated days. Viable cell numbers were determined by trypan blue exclusion and is expressed as the mean ± sd of three determinations. Control: Circle; GO-203: Square; CP-2: Triangle. b-c BALB/c nu/nu mice were injected subcutaneously in the flank with 1 × 107 COLO-205 (b) or SK-CO-1 (c) cells. The mice were pair matched when the tumors were ~100 mm3. b Treatment groups consisted of 8 mice injected intraperitoneally (ip) with PBS (vehicle control) (Square), 18 mg/kg GO-203(Circle) or 18 mg/kg CP-3 (Triangle) each day for 28 days. c Treatment groups consisted of 8 mice injected iv with PBS, 6 mg/kg GO-203 each day for 5 days, or 3 mg/kg GO-203 each day for 5 days each week for 2 weeks. Tumor measurements were performed as indicated and mice were weighed twice weekly. There was no weight loss in any of the groups. d Harvested tissues were stained for TIGAR expression by immunohistochemistry. e Schema depicting the proposed mechanism. * p < 0.05, ** p < 0.01, *** p < 0.001 as compared to control

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