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Table 1 Primer sequences used in the study

From: Functional role of ALK-related signal cascades on modulation of epithelial-mesenchymal transition and apoptosis in uterine carcinosarcoma

Assay Gene/region   Sequence
Promoter ALK −2056 Forward 5'-GCTCGCTAGCCTCGAACTGTGTGATGTGTTAG-3'
−1456 Forward 5'-GCTCGCTAGCCTCGATGAGATAATTCTTTG-3'
−956 Forward 5'-GCTCGCTAGCCTCGATGAGTTCTGTGTTGG-3'
−416 Forward 5'-GCTCGCTAGCCTCGAAGTCGGACCCGTTTA-3'
−146 Forward 5'-GCTCGCTAGCCTCGAAGGCCGGACTGCGTG-3'
+30 Reverse 5'-TCTTGATATCCTCGAGTACCAGCTGCTACC-3'
N-myc −221Forward 5'-CTCGCTAGCCTCGCAGCAGCTTTGCAGCCTTCTC-3'
+1312 Reverse 5'-AACCAGGTTCCCCAATCTTC-3'
Twist 1 −1086 Forward 5'-GCGTATCCAAGCATTTGGAATTGGGG-3'
−601Foward 5'-CCCAGGACCTCCGGGCTGGG-3'
−101 Forward 5'-GGGGACTGGAAAGCGGAAAC-3'
+101 Forward 5'-GCGTCCAGCCGTTGGGCGCT
+350 Reverse 5'-CTCTCGAGCGGCGACGCGTGGCCTC-3'
Mutagenesis ALK E-box1 Forward 5'-GCTGTATAGTGGCGGGCGCCCAGGCAG-3'
Reverse 5'-GCCCGCCACTATACAGCTGGCTGAGCCGCGC-3'
ALK E-box2 Forward 5'-CAGGTATAGTGCGATCCAGCGGCTCTG-3'
Reverse 5'-GGATCGCACTATACCTGGGCGCCCGCCACTT-3'
shRNA sh2969 Forward 5'-GATCCCGAATATTAAGCATTATCTAAAGCT
TCCTGTCACTTTAGATAATGCTTAATATTCTTTTTTG-3'
Reverse 5'-AATTCAAAAAAGAATATTAAGCATTATCTA
AAGTGACAGGAAGCTTTAGATAATGCTTAATATTCGG-3'
sh2386 Forword 5'-GATCCCGTACAAACCAGTTAATCCAGAGCT
TCCTGTCACTCTGGATTAACTGGTTTGTACTTTTTTG-3'
Reverse 5'-AATTCAAAAAAGTACAAACCAGTTAATCCA
GAGTGACAGGAAGCTCTGGATTAACTGGTTTGTACGG-3'
ChIP ALK −126 Forword 5'-GCGGAGTTGGCTTGTGAGCC-3'
+12 Reverse 5'-TGCTACCACCGCTGCCGCC-3'
Twist 1 −101 Forward 5'-GGGGACTGGAAAGCGGAAAC-3'
+62 Reverse 5'-TGCAGAGCCCGCGAGGTGT-3'
mRNA Twist1 Forward 5'-ATGATGCAGGACGTGTCCAGC-3'
Reverse 5'-CTAGTGGGACGCGGACATGG-3'
N-myc Forward 5'-TTCTCACGCTCAGGGACCACGT-3'
Reverse 5'-GAAGCGTCTAGCAAGTCCGAGC-3'
Slug Forward 5'-ACGCAATCAATGTTTACTCG-3'
Reverse 5'-TGAAGAGAAAGGTTACTGTC-3'
Snail Forward 5'-TGCCTCGACCACTATGCCGC-3'
Reverse 5'-AGCATTGGCAGCGAGGCGGT-3'