Table 1 Primer sequences used in the study
Assay | Gene/region | Sequence | |
|---|---|---|---|
Promoter | ALK | −2056 Forward | 5'-GCTCGCTAGCCTCGAACTGTGTGATGTGTTAG-3' |
−1456 Forward | 5'-GCTCGCTAGCCTCGATGAGATAATTCTTTG-3' | ||
−956 Forward | 5'-GCTCGCTAGCCTCGATGAGTTCTGTGTTGG-3' | ||
−416 Forward | 5'-GCTCGCTAGCCTCGAAGTCGGACCCGTTTA-3' | ||
−146 Forward | 5'-GCTCGCTAGCCTCGAAGGCCGGACTGCGTG-3' | ||
+30 Reverse | 5'-TCTTGATATCCTCGAGTACCAGCTGCTACC-3' | ||
N-myc | −221Forward | 5'-CTCGCTAGCCTCGCAGCAGCTTTGCAGCCTTCTC-3' | |
+1312 Reverse | 5'-AACCAGGTTCCCCAATCTTC-3' | ||
Twist 1 | −1086 Forward | 5'-GCGTATCCAAGCATTTGGAATTGGGG-3' | |
−601Foward | 5'-CCCAGGACCTCCGGGCTGGG-3' | ||
−101 Forward | 5'-GGGGACTGGAAAGCGGAAAC-3' | ||
+101 Forward | 5'-GCGTCCAGCCGTTGGGCGCT | ||
+350 Reverse | 5'-CTCTCGAGCGGCGACGCGTGGCCTC-3' | ||
Mutagenesis | ALK E-box1 | Forward | 5'-GCTGTATAGTGGCGGGCGCCCAGGCAG-3' |
Reverse | 5'-GCCCGCCACTATACAGCTGGCTGAGCCGCGC-3' | ||
ALK E-box2 | Forward | 5'-CAGGTATAGTGCGATCCAGCGGCTCTG-3' | |
Reverse | 5'-GGATCGCACTATACCTGGGCGCCCGCCACTT-3' | ||
shRNA | sh2969 | Forward | 5'-GATCCCGAATATTAAGCATTATCTAAAGCT |
TCCTGTCACTTTAGATAATGCTTAATATTCTTTTTTG-3' | |||
Reverse | 5'-AATTCAAAAAAGAATATTAAGCATTATCTA | ||
AAGTGACAGGAAGCTTTAGATAATGCTTAATATTCGG-3' | |||
sh2386 | Forword | 5'-GATCCCGTACAAACCAGTTAATCCAGAGCT | |
TCCTGTCACTCTGGATTAACTGGTTTGTACTTTTTTG-3' | |||
Reverse | 5'-AATTCAAAAAAGTACAAACCAGTTAATCCA | ||
GAGTGACAGGAAGCTCTGGATTAACTGGTTTGTACGG-3' | |||
ChIP | ALK | −126 Forword | 5'-GCGGAGTTGGCTTGTGAGCC-3' |
+12 Reverse | 5'-TGCTACCACCGCTGCCGCC-3' | ||
Twist 1 | −101 Forward | 5'-GGGGACTGGAAAGCGGAAAC-3' | |
+62 Reverse | 5'-TGCAGAGCCCGCGAGGTGT-3' | ||
mRNA | Twist1 | Forward | 5'-ATGATGCAGGACGTGTCCAGC-3' |
Reverse | 5'-CTAGTGGGACGCGGACATGG-3' | ||
N-myc | Forward | 5'-TTCTCACGCTCAGGGACCACGT-3' | |
Reverse | 5'-GAAGCGTCTAGCAAGTCCGAGC-3' | ||
Slug | Forward | 5'-ACGCAATCAATGTTTACTCG-3' | |
Reverse | 5'-TGAAGAGAAAGGTTACTGTC-3' | ||
Snail | Forward | 5'-TGCCTCGACCACTATGCCGC-3' | |
Reverse | 5'-AGCATTGGCAGCGAGGCGGT-3' |