Fig. 6
From: ITIH5 mediates epigenetic reprogramming of breast cancer cells
Epigenomic reprogramming of genes involved in cell adhesion and proliferation triggered by ITIH5 expression. Analysis of the DNA methylation profiles of the MDA-MB-231 WT, mock (#1 and #2), and ITIH5 transfected MDA-MB-231 ITIH5 single-cell clones (4, 7, and #12) using the Infinium Human Methylation450 (450 K) BeadChip technology. a Heatmap of 1512 CpG sites exhibiting significant (p < 0.05, Δβ-value > 0.2) methylation alterations between MDA-MB-231 WT, mock and ITIH5 single-cell clones demonstrated an epigenetic reprogramming of MDA-MB-231 cancer cells. b Cartoon illustrating gene ontology analysis of annotated 695 genes corresponding to the 1511 CpG sites. c Heatmap analysis of 242 hyper- or hypomethylated CpG sites (out of identified 1511 CpGs) located in potential regulatory promoter regions that are associated with H3K4Me3 and/or H3K27Me3 enrichment in a 5000 bp region upstream and downstream from the TSS. Chromatin status: K27 = H3K27Me3, K4 = H3K4Me3