Fig. 1

Myeloma tumor burden is increased in MMP-7-deficient mice. Wild-type (WT) or MMP-7 homozygous deficient (MMP-7^−/−) mice were inoculated with 5TGM1 myeloma cells (MM) or vehicle control. Tumor burden was measured by (a) serum IgG2bκ concentrations and (b) flow cytometric analysis of GFP-positive myeloma cells in the bone marrow and spleen. c Myeloma cell proliferation in the bone marrow was quantitated by phospho-histone H3 staining, (d) Myeloma cell apoptosis was quantitated by TUNEL staining. Data represent mean ± SEM. Mann Whitney U test *p < 0.05, **p < 0.01, ***p < 0.001 as compared to WT + MM. (WT, n = 7; MMP-7^−/−, n = 4; WT + MM, n = 8; MMP-7^−/− + MM, n = 5)