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Fig. 1 | Molecular Cancer

Fig. 1

From: BMI1, a new target of CK2α

Fig. 1

Expression and regulation of BMI1 and CK2α in ovarian cancer cells. a Slow migrating phospho-BMI1 bands were reduced upon treatment with λ-Phosphatase but not with AKT inhibitor. OV90 and CP20 cells were treated with 5 μM AKT inhibitor (AKTi) or vehicle (C) for 15 h. Protein extracted from the vehicle treated cells (C) were further subjected to λ-phosphatase (λ-P) treatment or left untreated. Expression of BMI1 and Phospho-AKT (Serine 473) were determined in the C, λ-P and AKTi treated cells. α-Tubulin and AKT were used as a loading control. Slow migrating BMI1 bands that were removed on phosphatase treatment is considered as the phospho species of BMI1 and is indicated by the open arrow head whereas the basal BMI1 is indicated by the closed arrow head. b Expression of BMI1, CK2α, and AKT (S473) in a panel of ovarian cancer cells. Protein expression was determined in 4 cell lines representing high grade serous ovarian cancer (TYKNU, OVSAHO and OV90) or cisplatin resistant CP20 and compared to the normal Fallopian Tube Epithelial Cells (FTE188). α-Tubulin and AKT served as a loading. Open and closed arrow heads represent phospho species of BMI1 and basal BMI1 respectively. c-d Effect of CK2α knockdown on BMI1. c OV90 and CP20 cells were either transfected with scrambled siRNA (siCTL) or siRNA against the CK2α gene (siCK2α) and expression of BMI1, RING1A and CK2α were determined by immunoblotting. d Relative mRNA expression of BMI1 and CK2α were determined by RT-qPCR. Gene expression of 18 s rRNA was used as an endogenous control. Values represent mean fold change (± standard deviation) over control (siCTL). Open and closed arrow heads represent phospho species of BMI1 and basal BMI1 respectively. e Effect of CK2α overexpression on BMI1. FTE188 cells were transfected with CK2α plasmid and 24 h post transfection the expression of BMI1 and CK2α were determined by immunoblotting. Open and closed arrow heads represent phospho species of BMI1 and basal BMI1 respectively

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