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Fig. 5 | Molecular Cancer

Fig. 5

From: Mechanistic added value of a trans-Sulfonamide-Platinum-Complex in human melanoma cell lines and synergism with cis-Platin

Fig. 5

Cellular distribution of CDKN1A and CDKN1B in melanoma cell lines and its alteration after CDDP or TSPC treatment. a Subcellular distribution of CDKN1A and CDKN1B in SK-MEL-5 and SK-MEL-28 cells, after CDDP and TSPC treatment assessed by confocal microscopy. First and fourth columns: nuclei stained with DAPI; Second and fifth columns: CDKN1A (up) and CDKN1B (down) proteins stained with specific antibody and secondary antibody conjugated with Alexa Fluor 488; Third and sixth columns: colocalization. b Quantitative analysis of nuclear CDKN1A (left) and CDKN1B (right) staining in melanoma cell lines. Staining cells were counted at 200 × magnification from six to ten randomly selected fields. Total 100 cells were counted in each experiment. (*, p < 0.05)

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