Fig. 2From: Vasohibin 2 reduces chemosensitivity to gemcitabine in pancreatic cancer cells via Jun proto-oncogene dependent transactivation of ribonucleotide reductase regulatory subunit M2VASH2 decreases the sensitivity of pancreatic cancer cells to gemcitabine in vitro. After treatment with 25 or 50 nM gemcitabine for 48 h, the apoptosis rate of PANC-1-EGFP, PANC-1-VASH2, SW1990-scramble, and SW1990-shVASH2 was indicated by flow cytometric determination of Annexin V and 7-AAD staining. Upper-right + lower-right quadrants indicate apoptotic cells. Apoptosis is quantified in the bar graphs for PANC-1-EGFP and PANC-1-VASH2 (top) and SW1990-scramble and SW1990-shVASH2 (bottom). *P < 0.05Back to article page