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Fig. 4 | Molecular Cancer

Fig. 4

From: Vasohibin 2 reduces chemosensitivity to gemcitabine in pancreatic cancer cells via Jun proto-oncogene dependent transactivation of ribonucleotide reductase regulatory subunit M2

Fig. 4

VASH2 regulates the expression of RRM2 and VASH2 expression correlates with RRM2 expression in human pancreatic cancer. a human gene expression NimbleGen 12x135K microarrays within PANC-1-EGFP/PANC-1-VASH2 cells. Fold changes ≥ 3 (upregulation; 211 genes) or ≤ 0.33 (downregulation, 192 genes) were selected as significantly altered genes for subsequent analysis. RRM2 (a typical gemcitabine resistance associated gene) was upregulated by VASH2 (fold change = 3.27). b and c qPCR analysis and immunobloting test of RRM2 in PANC-1-VASH2, PANC-1-EGFP cells, SW1990-shVASH2 and SW1990-scramble cells. d representative images of RRM2 and VASH2 staining in human pancreatic cancer tissues expressing low (a) and high (b) levels of VASH2 are shown. (Spearman’s rank correlation coefficient, r = 0.41, P = 0.001). *P < 0.05

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