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Fig. 5 | Molecular Cancer

Fig. 5

From: Regulation of stem-like cancer cells by glutamine through β-catenin pathway mediated by redox signaling

Fig. 5

Decrease of SP subpopulation in A549 cells treated with hydrogen peroxide. a A549 cells were cultured without or with H2O2 (50 μM) for 48 h, cells were harvested and stained with Hoechst33342 to determine SP cells. b A549 cells were treated without or with H2O2 (50 μM) for 48 h, then RNA was isolated for real-time RT-PCR for analysis of expression of ALDH-1 and SOX-2. β-actin was used as an internal control. c A549 cells was treated with 5 mM aminotriazole (ATZ) for 72 h with or without pretreatment with NAC, cell lysates were subjected to western blotting to measure the expression of ABCG2 and SOX-2. d A549 cells were treated with 5 mM aminotriazole (ATZ) for 48 h, cells were harvested and stained with Hoechst 33342 to determine SP cells. e A549 cells was treated with glutamine-free medium for 72 h with or without pre-treatment of 2 mM NAC, the cells were collected for SP detection. **, p < 0.01

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