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Fig 4 | Molecular Cancer

Fig 4

From: MDM4 actively restrains cytoplasmic mTORC1 by sensing nutrient availability

Fig 4

MDM4 interacts with cytoplasmic mTOR. a Representative pictures of immunofluorescence of HeLa cells transiently transfected with MDM4 and after 24 h treated with EBSS for 50’ and with aa for the last 15’. Endogenous mTOR is stained in green, MDM4 in red. DNA in blue (by DAPI). Merge shows the overlap of the signals. b Representative pictures of immunofluorescence of HeLa cells transfected with siCTL or siMDM4 and after 48 h treated with EBSS for 50’ and with aa for the last 15’. Endogenous mTOR is stained in green, DNA in blue (by DAPI). Merge shows the overlap of the signals. c Percentage of cells showing mTOR punctuated staining. Mean ± SD of three independent biological replicates is shown (** = p < 0.01; *** = p < 0.001 two‐tailed unpaired t‐test). d Wb analysis of the indicated proteins in HeLa cells transfected with siMDM4 or siCTL. After 48 h, cell lysates were fractionated in light membrane/cytosol (lm/cyt) and heavy membrane (hm) fractions. Left panel shows WCE. Histogram shows the percentage (%) of mTOR signal in the lm (light bar) and hm (black bar) fractions corrected for the respective loading control. mTOR signal in the lm + hm fractions was arbitrarily set to 100%

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