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Fig 5 | Molecular Cancer

Fig 5

From: MDM4 actively restrains cytoplasmic mTORC1 by sensing nutrient availability

Fig 5

MDM4 regulates cell size and cell proliferation in a mTOR-dependent manner. a, b Forward scatter analysis (FSC-H) of p53−/−HCT116, p53+/+HCT116 (a) and MCF10A cells (b) transfected with siMDM4 or siCTL and after for 48 h analysed by flow cytometry. Mean ± SD of three independent biological replicates is shown (N = 3). c FSC-H of p53 −/−MEFs and p53 −/− Mdm4 −/−MEFs (N = 3). d FSC-H of 293 T cells transfected with siMDM4 or siCTL and after 24 h treated with RAPA (40nM) for additional 24 h (N = 3). e Cell viability by Cell Titer Blue colorimetric assay of 293 T cells treated as in (d) (N = 3). f Evaluation of viable cells by Trypan blue in 293 T cells transfected with siMDM4 or siCTL and after 24 h treated with RAPA or transfected with siS6K1 for additional 24 h (N = 3, * = p < 0.05, ** = p < 0.01, two‐tailed unpaired t‐test)

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