Fig. 2

Upregulation of GPR87 expression promotes pancreatic cancer cell aggressiveness in vitro. a. Western blot of GPR87 expression in PANC-1 and AsPC-1 cells stably overexpressing GPR87. α-tubulin was used as a loading control. b. Representative images (left panel) and quantification (right panel) of colonies in an anchorage-independent growth assay. Colonies larger than 0.1 mm in diameter were scored. c. Representative micrographs (left panel) and quantification (right panel) of BrdU labeling in cells transfected with GPR87 or a vector control. d. Representative images (left panel) and quantification (right panel) of HUVECs cultured on matrigel-coated plates with conditioned medium from vector control or GPR87-transduced pancreatic cancer cells. e. Representative images of CAM blood vessels stimulated with conditioned medium from the indicated cells. f. Representative images (upper panel) and quantification (lower panel) of invading cells based on a transwell matrix penetration assay. g. Representative images (left panel) and quantification (right panel) of Annexin V-FITC and PI staining of PANC-1 and AsPC-1 cells overexpressing GPR87 treated with gemcitabine (1 μM) for 24 h. Each bar represents the mean ± SD of three independent experiments. * p < 0.05