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Fig. 3 | Molecular Cancer

Fig. 3

From: Tumor-derived CXCL5 promotes human colorectal cancer metastasis through activation of the ERK/Elk-1/Snail and AKT/GSK3β/β-catenin pathways

Fig. 3

CXCL5 localization in CRC tissues, the expression of CXCL5 in CRC cell lines and CXCL5/CXCR2 promotes CRC cell migration through the induction of EMT. a & b Immunohistochemistry and immunofluorescence images showing the location of CXCL5 and αSMA (marker of fibroblasts) in CRC specimens. CXCL5 is mainly expressed in tumor tissue instead of mesenchymal tissue. c Immunoblots examining CXCL5 expression levels in CRC cell lines and normal colon epithelial cell. d Representative data from the immunoblot analysis verifying the changes of CXCL5 or CXCR2 using a CXCL5-vector or shRNA-inhibition of CXCL5 and CXCR2. e & f Representative images from the transwell migration assay in indicated CRC cells. Magnification, 200×. Scale, 200 μm. Data represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. g Immunoblot analysis of EMT marker expression in indicated cells. h Immunofluorescence images showing the changes in E-cadherin and Vimentin after different treatments with CXCL5-vector or shRNA-inhibition of CXCL5 and CXCR2. Scale, 50 μm

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Molecular Cancer

ISSN: 1476-4598