Skip to main content
Fig. 5 | Molecular Cancer

Fig. 5

From: Tumor-derived CXCL5 promotes human colorectal cancer metastasis through activation of the ERK/Elk-1/Snail and AKT/GSK3β/β-catenin pathways

Fig. 5

Snail is critical to CXCL5/CXCR2-mediated CRC migration. a Immunoblot analysis of Snail, Slug and ZEB1 levels in the indicated cells. CXCL5 is able to induce Snail expression. b Inhibition of the ERK pathway (U0126) instead of the AKT pathway (LY294002) downregulates Snail expression in the indicated cells. c Downregulation of Snail inhibits CXCL5-induced downregulation of E-cadherin and upregulation of Vimentin and N-cadherin in HCT116. Downregulation of Snail upregulates E-cadherin expression and downregulates Vimentin and N-cadherin expression in SW480. d Inhibition of Snail altered the morphology of cells in both the HCT116CXCL5 and SW480 groups. e & f Inhibition of Snail decreased the number of migrating cells in the indicated cell lines. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. g & h Immunofluorescence images show that downregulation of Snail inhibits CXCL5-induced downregulation of E-cadherin and upregulation of Vimentin and N-cadherin in HCT116. Downregulation of Snail upregulates E-cadherin expression and downregulates Vimentin and N-cadherin expression in SW480. Scale, 50 μm

Back to article page