Fig. 3

MSLN knockdown reduces mesenchymal like morphology, cell-substrate adhesion, cell migration and invasion. a Representative images of cell morphology of shC and shMSLN clones. b Digital quantification of the cell surface area using image J software, means ± s.d., *P < 0.05 vs shC. c Average size of shC and shMSLN cells from flow cytometry FSC-A median. d Representative images of fluorescently labeled cells before and after washing to remove non-adherent cells. Green fluorescent shC cells and red fluorescent shMSLN cells. e Quantification of the relative number of attached cells after washing normalized by the number before washing, *P < 0.05 vs shC. f Representative images and g quantitation of migration of shC and shMSLN cells from Transwell, stained with Diff-Quik. h Representative images and i quantitation of invasion of shC and shMSLN cells from Matrigel-coated Transwell, stained with Diff-Quik. *P < 0.05 vs shC