Skip to main content
Fig. 6 | Molecular Cancer

Fig. 6

From: Mesothelin promotes epithelial-to-mesenchymal transition and tumorigenicity of human lung cancer and mesothelioma cells

Fig. 6

MSLN knockdown reduces EMT and cancer stem cell traits. a Relative fold change of gene expression from an EMT pathway specific PCR array. Data represent candidate transcripts exceeding two-fold up or down regulation in shC and shMSLN cells. b Western blot analysis of representative EMT and CSC markers. Actin was used a loading control. c Flow cytometry graphical representation of ALDH activity in shC and shMSLN cells with or without ALDH inhibitor DEAB. d Quantification of ALDH activity, means ± s.d. (n = 3), *P < 0.05. e Western blot analysis of MSLN expression in ALDH activity high (ALDG-H) and ALDH activity low (ALDH-L) fractions after flow cytometric ALDH sorting. f ALDH-H fraction has a relative higher number of anoikis resistant cells. Sorted ALDH-H and ALDH-L cells were seeded in low attachment plates and cell numbers were counted at 0 and 48 h, means ± s.d. (n = 3), *P < 0.05

Back to article page
\