Fig. 2

PinX1 expression in tissues obtained from two NSCLC cohorts and its prognostic significance in NSCLC patients. a Receiver operation characteristic curve analysis was employed to determine the cut-off score for the overexpression of PinX1. The sensitivity and specificity for each outcome were plotted. b Representative immunohistochemical images show the overexpression of PinX1 and low expression of PinX1 in a NSCLC tissue. high PinX1 expression in Lung Squamous Carcinoma (200×, 400×, upper right); high PinX1 expression in Lung Adenocarcinoma tissue (200×, 400×, lower right); low PinX1 expression in Lung Squamous Carcinoma tissue (200×, 400×, upper left); low PinX1 expression in Lung Adenocarcinoma tissue(200×, 400×, lower left). c High expression of PinX1 was associated with poor prognosis of NSCLC patients. Kaplan–Meier plots show the Overall Survival curves of 93 ESCC patients in the learning cohort and 51 NSCLC patients in the validation cohort, according to PinX1 expression levels in the primary tumor (p < 0.05, log-rank test). d PinX1 gene with alteration was correlated with Overall Survival and Disease-Free Survival in cBioportal Web resource online (Lung Adenocarcinoma – TCGA, Provisional)