Fig. 6

PinX1 associated with cell proliferation and cell cycle transition rely on BMP5 in NSCLC cell lines. a Western blotting was used to detect the expression of PinX1, BMP5, Bcl-2, Bax, P15^Inkand p-Rb in A549 PinX1-silenced and SK-MES-1-PinX1 cells treated with noggin (0.5 mg/ml) and BMP5 (10 μg/ml). b Cell viability treated with noggin (0.5 mg/ml) and BMP5 (10 μg/ml) was measured by MTT assay in A549 PinX1-silenced and SK-MES-1-PinX1 cells. c The percentage of S phase in A549 PinX1-silenced and SK-MES-1-PinX1 cells treated with noggin (0.5 mg/ml) and BMP5 (10 μg/ml) were determined by EdU cooperation assay. *, P < 0.05. d G1, G2, and S cell cycle phases in A549 PinX1-silenced and SK-MES-1-PinX1 cells treated with noggin (0.5 mg/ml) and BMP5 (10 μg/ml) were determined by flow cytometry assay. *, P < 0.05