Fig. 5

miR-34a is a tumor-suppressive miRNA. a Overexpressed miR-34a impaired GC cell growth in a 4-day MTT assay in MKN28 and SGC-7901 cells (**, P < 0.001). b Smaller sizes and less numbers of monolayer colonies were detected in miR-34a transfectants compared with negative control group (**, P < 0.001). c The cell invasion ability was partly abolished after treatment with miR-34a (**, P < 0.001). The invaded cells from the matrigel were counted in three random vision fields for getting standard deviations. d Cell cycle analysis by flow cytometry revealed that ectopic expression of miR-34a raised the proportion of G0/G1-phase cells. e Western blot analysis of related cell cycle regulators and apoptotic markers. f miR-34a overexpression in MGC-803 cells inhibited xenograft formation in vivo (**, P < 0.001)