Fig. 5
From: Phosphorylation by mTORC1 stablizes Skp2 and regulates its oncogenic function in gastric cancer
Skp2 S64 phosphorylated by mTOR is critical for gastric cancer cell proliferation. a BGC823 cells were transfected with the indicated plasmids for 48 h and harvested for Western blot analysis. (b, c) BGC823 cells with stable overexpression of Vector,WT-Skp2,Skp2 S64A or Skp2 S64D were plated for cell growth analysis (b) and soft agar transformation assay (c). Results are presented from a representative experiment. *p < 0.05, using Student’s t-test, n = 3. d BGC823 cells stably transfected with Vector,WT-Skp2,Skp2 S64A or Skp2 S64D were injected into nude mice (n = 5 for each group) for tumorigenesis. e Pictures were taken 7 weeks after the injection. *p < 0.05 using Student’s t-test