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Fig. 3 | Molecular Cancer

Fig. 3

From: Cripto-1 acts as a functional marker of cancer stem-like cells and predicts prognosis of the patients in esophageal squamous cell carcinoma

Fig. 3

CR-1 is associated with the invasion and metastasis of EC109 cells in vitro and in vivo. a Transwell chamber invasion assay revealed that the invasion capacity of CR-1high cells was stronger than that of the CR-1low cells. b qRT-PCR analysis of the expressions of E-cadherin and vimentin in CR-1 high and CR-1 low cells. c qRT-PCR analysis of differential expression of EMT-related transcription factors in CR-1 high and CR-1 low cells. d Silencing CR-1 expression significantly decreased the invasion of EC109 cells in vitro. e Wound healing experiments showed that CR-1 knockdown significantly reduced the capacity of migration in EC109 cells. f CR-1 knockdown suppressed the metastatic potential of EC109 cells in nude mice. g Western blot assay showed that down-regulation of CR-1 results in up-regulation of E-cadherin and down-regulation of vimentin, snail and MMP9 in EC109 cells. * indicates p < 0.05; *** indicates p < 0.001

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Molecular Cancer

ISSN: 1476-4598