Fig. 1

The effect of HOXA11-AS on gastric cancer cells proliferation and cell cycle progression. a qRT–PCR analysis of HOXA11-AS expression in BGC823, SGC7901 cells transfected with HOXA11-AS or NC siRNAs, and in AGS cells transfected with HOXA11-AS over-expression vector. b Growth curves of BGC823, SGC7901 and AGS cells after transfection with HOXA11-AS siRNAs or vector were determined by CCK8 assays. Values represented the mean ± s.e. from three independent experiments. c, d The cell cycle progression of BGC823 and SGC7901 cells was evaluated 48 h after transfection with HOXA11-AS siRNAs or NC using Flow cytometry assays. e The cell cycle progression of AGS cells was evaluated 48 h after transfection with HOXA11-AS vector or empty vector using Flow cytometry assays. The bar chart represented the percentage of cells in G0/G1, S, or G2/M phase, as indicated. f, g The cyclinD1, CDK2, CDK4 and CDK6 protein levels were detected in BGC823 and SGC7901 cells after transfection with HOXA11-AS siRNAs or NC using western blot. *P < 0.05, **P < 0.01