Fig. 5
From: Thymic Epithelial Tumors phenotype relies on miR-145-5p epigenetic regulation
Inhibition of HDAC activity reduces tumor characteristics of TET cells. a Representative cell cycle analysis of TC1889 cells after 72 h and 96 h of 3 mM VPA treatment. b Western-blot analysis to evaluate the expression levels of p21 after 72 h of 1 mM or 3 mM VPA treatment. c ATPlite analysis to evaluate the viability of TC1889 cell after 24 h of a dose-dependent VPA treatment. d Evaluation of the impact of 3 mM VPA treatment for 72 h on TC1889 colony forming capability. The experiment was performed in triplicate and the graphical plot of the average (left panel) and a representative image (right panel) were reported. e Evaluation of the impact of 3 mM VPA treatment for 72 h on TC1889 migration capability. The experiment was performed in triplicate. f-g ATPlite analysis f and colony forming capability (g) of TC1889 cells treated for 48 h with the inhibitor of miR-145-5p (INH 145) or negative control (INH C) and subsequently treated with VPA 0.5 mM for additional 24 h. The experiment was performed in triplicate. P-value was calculated by unpaired t-test and a value of P ≤ 0.01(**), P ≤ 0.001(***) and P ≤ 0.0001(****) was considered statistically significant