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Fig. 5 | Molecular Cancer

Fig. 5

From: HN1 contributes to migration, invasion, and tumorigenesis of breast cancer by enhancing MYC activity

Fig. 5

HN1 regulates MYC expression. a Enrichment plot, indicating a significant correlation between HN1 mRNA level and the MYC regulated gene signatures (*P <0.05). b Correlation of MYC and HN1 expression (P = 0.044). c Western blot analysis of the expression of HN1 and MYC in indicated cells with HN1 overexpression or knockdown, β-actin was used as the loading control. d The heatmap for the expression of MYC targeted genes including CDK4, CCND1, p21, CAV1, and SFRP1 after overexpression or knockdown of HN1. Quantitative real-time PCR was used to determine their expression. e Western blot analyzed the expression of CDK4, Cyclin D1 and p21 after HN1 overexpression or knockdown. GAPDH was used as the loading control. f Luciferase assay of MYC activity after overexpression or knockdown of HN1(*P <0.05). Each bar represents mean ± SD of 3 independent experiments

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