Fig. 2
From: PERK induces resistance to cell death elicited by endoplasmic reticulum stress and chemotherapy
Expression of MRP1 in cells resistant to chemotherapy and to ER stress. a. Immunoblots of the indicated proteins in extracts of untreated cells. β-tubulin was used as a loading control. The figure is representative of 3 experiments with similar results. b. MRP1 mRNA level as measured by qRT-PCR. Data are mean ± SD (n = 4). *p < 0.001 vs. HT29 cells. c. Representative flow cytometry histograms of MRP1 protein. Grey peaks: non immune isotypic antibody. d. Cell surface MRP1 was determined by flow cytometry. Data are mean fluorescence intensity (MFI) ± SD (n = 3). *p < 0.02 vs. HT29 cells. e. Intracellular doxorubicin content, an index of MRP1 activity, measured by fluorimetry. Data are mean ± SD (n = 3). *p < 0.001 vs. HT29 cells