Fig. 6
From: MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway
Down-regulation of FLOT1 phenocopied the effect of miR-608. a CCK-8 assay. The relative viabilities of T24 and UM-UC-3 cells transfected with siFLOT1 were significantly lower than NC treated cells. b Colony-formation assay (Representative wells were presented). The colony formation rates of siFLOT1 transfected BCa cells were sharply lower in contrast with NC transfected cells. c Flow cytometric analysis of cell cycle distribution. Knock-down of FLOT1 induced the similar G1 arrest as miR-608 did in BCa cells. d Western blot analysis. siFLOT1 down-regulated AKT/FOXO3a signaling related proteins in T24 and UM-UC-3 cell lines. e The protein and mRNA levels of FLOT1 were significantly down-regulated by the transfection of siFLOT1. f and g qRT-PCR analysis of the expression of CCND1 and CDK4 mRNA in BCa cells after the transfection of siFLOT1. Error bars represent the S.D. from three independent experiments. *P < 0.05