Table 1 The oligonucleotides used in this study
From: MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway
Namea | Sequence (5′- > 3′)b |
|---|---|
miR-608 mimics (Sense) | AGGGGUGGUGUUGGGACAGCUCCGU |
NC (Sense) | ACUACUGAGUGACAGUAGA |
miR-608 Inhibitor | ACGAGCUGUCCCAACACCACCCCU |
Inhibitor NC | CAGUACUUUUGUGUAGUACAA |
miR-608Â F | AGGGGTGGTGTTGGGACAGCTCCGT |
miR-608 probec | ACGGA GCTGT CCCAA CACCA CCCCT |
U6 F | TGCGGGTGCTCGCTTCGGCAGC |
CDK4 F | ATGGCTACCTCTCGATATGAGC |
CDK4 R | CATTGGGGACTCTCACACTCT |
CCND1 F | GCTGCGAAGTGGAAACCATC |
CCND1 R | CCTCCTTCTGCACACATTTGAA |
FLOT1 F | CCCATCTCAGTCACTGGCATT |
FLOT1 R | CCGCCAACATCTCCTTGTTC |
GAPDH F | AAGGTGAAGGTCGGAGTCA |
GAPDH R | GGAAGATGGTGATGGGATTT |
FLOT1 UTR Wt-1Â F | CTGTCCATTGACAGTGAGGTCCCACCCCTCATCTCTCCTTGCCAAATAG |
FLOT1 UTR Wt-1 R | TCGACTATTTGGCAAGGAGAGATGAGGGGTGGGACCTCACTGTCAATGGACAGAGCT |
FLOT1 UTR Mut-1Â F | CTGTCCATTGACAGTGAGGTCGGTGGGGTCATCTCTCCTTGCCAAATAG |
FLOT1 UTR Mut-1 R | TCGACTATTTGGCAAGGAGAGATGACCCCACCGACCTCACTGTCAATGGACAGAGCT |
FLOT1 UTR Wt-2Â F | CAGCCTTCTGATGATCCCACTCCACCCCACCTCAACTTATTTAACTTCG |
FLOT1 UTR Wt-2 R | TCGACGAAGTTAAATAAGTTGAGGTGGGGTGGAGTGGGATCATCAGAAGGCTGAGCT |
FLOT1 UTR Mut-2Â F | CAGCCTTCTGATGATCCCACTGGTGGGGACCTCAACTTATTTAACTTCG |
FLOT1 UTR Mut-2 R | TCGACGAAGTTAAATAAGTTGAGGTCCCCACCAGTGGGATCATCAGAAGGCTGAGCT |