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Fig. 4 | Molecular Cancer

Fig. 4

From: Blockade of adenosine A2A receptor enhances CD8+ T cells response and decreases regulatory T cells in head and neck squamous cell carcinoma

Fig. 4

A2AR is upregulated on Tregs and CD8+ T cells in 2cKO tumor bearing mice. a Representative image of Foxp3 immunohistochemical staining with quantification in normal tongue mucosa from WT mice and HNSCC from 2cKO tumor bearing mice (each group n = 6, scale bar = 100 μm, Mean ± SEM, ***, P < 0.001, unpaired t test). b The gating strategy of A2AR expression on CD4+ Foxp3+ Tregs. c Representative dot plots of CD4+ Foxp3+ Tregs in spleen of WT mice and 2cKO tumor bearing mice (left). Quantitative analysis of the CD4+ Foxp3+ Tregs population in spleen, lymph nodes, and peripheral blood of 2cKO tumor bearing mice as compared with WT mice (right, each group n = 6, Mean ± SEM, *, P < 0.05, **, P < 0.01, unpaired t test). d Representative dot plots of A2AR expression on CD4+ Foxp3+ Tregs in WT mice and 2cKO tumor bearing mice (left). Quantitative analysis of the frequency of A2AR expression on CD4+ Foxp3+ Tregs in spleen, lymph node, and peripheral blood of WT mice and 2cKO tumor bearing mice (right, each group n = 6, Mean ± SEM, *** P < 0.001, unpaired t test). e Representative dot plots of A2AR expression on CD8+ T cells in WT mice and 2cKO tumor bearing mice (left). Quantitative analysis of the frequency of A2AR expression on CD8+ T cells in spleen, lymph node, and peripheral blood of WT mice and 2cKO tumor bearing mice (right, each group n = 6, Mean ± SEM, *** P < 0.001, unpaired t test)

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