Fig. 6

A2AR blockade decreases CD4⁺ Foxp3⁺ Tregs in HNSCC mouse model. a Representative photos of Foxp3 immunohistochemical staining of vehicle group and SCH58261 treated group (left). The histoscore quantification of Foxp3 was showed by bar graph (right, each group n = 6, Mean ± SEM, ***, P < 0.001, unpaired t test). b Representative dot plots of CD4⁺ Foxp3⁺ Tregs in spleen of Vehicle group and SCH58261 group (left). Bar graphs showed quantitative analysis of the percentage of CD4⁺ Foxp3⁺ Tregs in spleen, lymph node, peripheral blood and tumor from each group (right, each group n = 6, Mean ± SEM, *, P < 0.05, **, P < 0.01, unpaired t test). c Representative dot plots of A2AR on CD4⁺ Foxp3⁺ Tregs in spleen of mice from vehicle group and SCH58261 group (left). Quantification of the frequency of A2AR on CD4⁺ Foxp3⁺ Tregs in each group is presented by bar graph (right, each group n = 6, Mean ± SEM, *, P < 0.05, **, P < 0.01, unpaired t test)