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Fig. 1 | Molecular Cancer

Fig. 1

From: ROS production induced by BRAF inhibitor treatment rewires metabolic processes affecting cell growth of melanoma cells

Fig. 1

BRAF inhibitors induce upregulation of PDH-E1α phosphorylation in BRAFV600E melanoma cells but not in wild-type BRAF melanoma cells. Western blot analysis of A375, IGR37 and 501 Mel cells (BRAFV600E) (a) and MelJuso, IPC298 and SKMel30 cells (BRAFWT/NRASmut) (b) treated with 1 μM of PLX4032 and 100 nM of GSK2118432 for the indicated time points. α-Tubulin was used as loading control; representative blots of three biological replicates are shown. c Quantification of pPDH and PDK1 levels for A375, IGR37 and 501Mel, normalized to the untreated control. Error bars represent the standard deviation of three biological replicates. Statistical significance was determined using one-way ANOVA coupled with Dunnett’s multiple comparisons tests. *p > 0.05, **p > 0.01, ***p > 0.001

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