Fig. 3
PLX4032 treatment induces upregulation of PDK mRNA in BRAFV600E melanoma cells. Quantitative RT-PCR of PDKs and HIF-1α mRNA. The fold change was calculated relative to untreated controls. Error bars represent the standard deviation of three biological replicates. PDK2 was not detectable in tested cell lines (Cq ≥ 30) while PDK4 (Cq ≥ 30) was not detectable in IGR37 cells only. Error bars represent the standard deviation of three biological replicates. Statistical significance was determined in comparison to the untreated control using paired Student’s t-tests. *p > 0.05, **p > 0.01, ***p > 0.001