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Fig. 1 | Molecular Cancer

Fig. 1

From: Clonal competition in BcrAbl-driven leukemia: how transplantations can accelerate clonal conversion

Fig. 1

Vector construction and experimental set-up. a Wildtype BcrAbl (p210) in conjunction with eGFP was cloned into the γ-retroviral vector MP91 [21, 22]. The viral backbone was additionally equipped with a truncated sequence of Illumina Adaptors and our BC32 construct [23]. 1010 plasmids of the obtained plasmid library were used for next-generation sequencing via Miseq (Illumina) and >80,000 different plasmids were obtained. b BcrAbl-barcode containing viral particles were used to transduce the IL-3-dependent murine hematopoietic cell line Ba/F3. After IL-3 withdrawal, the cells were kept in culture and every 2–4 days an aliquot of cells was analysed by FC and used for DNA extraction. Cells were sorted for eGFP on day 24 (i) and on day 45 (ii) (after thawing). eGFP-positive cells were taken and transplanted into non-conditioned female Balb/C mice on day 29 (i) (1000 or 10,000 cells per mouse) and on day 49 (ii) (10,000 cells per mouse)

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