Fig. 2

miR-455-3p enhances ESCC chemoresistance and tumorigenesis. a miRNA array (left) and real-time PCR (right) analyses of miR-433-5p in the indicated cells. Microarray data accession number is GSE83362. Transcript levels were normalized to U6 expression. b GSEA of TCGA datasets indicating that miR-455-3p expression was significantly correlated with chemoresistance gene signatures. c Representative images of tumor-bearing mice (left), weight of xenografts (middle), and quantification of TUNEL-stained cells (right) in the indicated tumors. d Representative images (left) and quantification (right) of tumorspheres formed by the indicated cells. e Flow cytometry analysis of the percentages of CD90⁺/CD271⁺ subpopulations of the indicated cells. f The frequency (left) and estimated percentage (right) of T-ICs among the indicated cells during serial transplantations. Each bar represents the mean ± SD of three independent experiments. * P < 0.05