Fig. 2
The effects of HOXD-AS1 on metastasis in vitro and in vivo. a-c The effects of HOXD-AS1 on HCC cancer cell metastasis were determined by using the Millipore Transwell chambers. Representative images of the bottom surfaces of the chambers with different HCC cells are shown. d Scratch wound healing experiments in HOXD-AS1-overexpressing or -silenced HCCLM3 cells. To evaluate the pro-metastatic potential of HOXD-AS1 in vivo, the firefly luciferase-labelled HCCLM3 cells were infected with HOXD-AS1-overexpressing or control lentiviruses for further bioluminescence imaging. e The luciferase signal intensities of the mice were examined four weeks after intraspleen injection with 2 × 106 HCCLM3 cells. f The luciferase intensity was analyzed in six mice in each group. g The luciferase signal intensities of the mice were examined at the indicated time points after tail vein injection with 2 × 106 HCCLM3 cells. h The luciferase intensity was analyzed in six mice in each group. The data are shown as the mean ± SD, Student’s t test, *p < 0.05