Skip to main content
Fig. 6 | Molecular Cancer

Fig. 6

From: The noncoding RNA HOXD-AS1 is a critical regulator of the metastasis and apoptosis phenotype in human hepatocellular carcinoma

Fig. 6

HOXD-AS1 downregulated RGS3 expression and inhibited Dox-induced apoptosis. a The protein expression levels of cleaved caspase9, cleaved caspase3 and cleaved PARP were determined by westernblot analysis after Dox treatment. b Quantification by westernblot of phosphorylated ERK and MEK in HCCLM3 cells when HOXD-AS1 was overexpressed compared to pcDNA3.1 empty vector. c and d Quantification of RGS3 mRNA expression by QRT-PCR when HOXD-AS1 was overexpressed or silenced by siRNA. e HCCL3 cells were treated with siRNA targeting HOXD-AS1 or RGS3 followed by examination of Dox-induced apoptosis by flow cytometry. f MTT assays were performed after siRNA (siNC, si-HOXD-AS1 or si-RGS3) treatment. g Schematic model of HOXD-AS1 functions during liver cancer progression

Back to article page

Molecular Cancer

ISSN: 1476-4598