Fig. 1

linc00152 functioned as an oncogene in GSCs. a Expression of linc00152 in glioma tissues of different grades and normal brain tissues (NBTs). (Data are presented as the mean ± SD (n = 10, each group). **P < 0.01 vs. NBTs group; *P < 0.05 vs. low grade group; ^#P < 0.05 vs. low grade group. b Expression of linc00152 in non-GSCs and GSCs (Data are presented as the mean ± SD (n = 5, each group). **P < 0.01 vs. non-GSCs-U87 group; ^##P < 0.01 vs. non-GSCs-U251 group. c Relative expression of linc00152 after GSCs transfected with sh-linc00152 plasmids and scrambled vectors (NC). d CCK-8 assay was conducted to explore the proliferation effect of linc00152 on GSCs. e Limiting dilution assay showing the impact of linc00152 on the sphere-formation ability of GSCs. f Transwell assays for assessing GSC migration and invasion. Typical images and accompanying statistical plots were presented. g Flow cytometry detected the apoptosis of GSCs with the knockdown of linc00152. Data are presented as the mean ± SD (n = 5, each group). *P < 0.05 vs. sh-NC group. Scale bars represent 20 μm. The photographs were taken at 200 × magnification