Fig. 4

FEZF1 was a target gene of miR-103a-3p, the expression of FEZF1 was regulated by both linc00152 and miR-103a-3p. a qRT-PCR analysis of the effect of linc00152 on FEZF1 mRNA expression in glioma cells. **P < 0.01 vs. sh-NC group. b qRT-PCR analysis of the effect of miR-103a-3p on FEZF1 mRNA expression in glioma cells. **P < 0.01 vs. pre-NC group; ^##P < 0.01 vs. anti-NC group. c Western blot analysis on FEZF1 in linc00152-knockdown GSCs, with GAPDH as an endogenous control. *P < 0.05 vs. sh-NC group. d Western blot analysis for expression between miR-103a-3p and FEZF1 in GSCs, with GAPDH as an endogenous control. *P < 0.05 vs. pre-NC group; ^#P < 0.05 vs. anti-NC group. e Western blot analysis for FEZF1 in GSCs co-transfected with sh-linc00152 and pre-miR-103a-3p or anti-miR-103a-3p, with GAPDH as an endogenous control. *P < 0.05 vs. sh-NC+ pre-NC group. ^#P < 0.05 vs. sh-linc00152 + pre-NC group; ^&P < 0.05 vs. sh-NC + pre-miR-103a-3p group; ^▲P < 0.05 vs. sh-NC + anti-NC group; ^★P < 0.05 vs. sh-linc00152 + anti-NC group; ^▼P < 0.05 vs. sh-NC + anti-miR-103a -3p group. For a-e, data are presented as the mean ± SD (n = 5, each group). f Schematic representation of the putative binding site for miR-103a-3p and FEZF1, and the contrivable mutant sequence for the reporter assay. g The relative luciferase activities were hindered in the HEK-293 T cells co-transfected with vector pre-miR-103a-3p and FEZF1-wt. *P < 0.05. vs. FEZF1-wt + pre-NC group