Fig. 6

miR-103a-3p inhibited malignant progression of GSCs by binding to the FEZF1 3′-UTR. a Effect of miR-103a-3p and FEZF1 co-transfection on the CDC25A mRNA expression. b The CCK-8 assay was used to assess the effects of miR-103a-3p and FEZF1 on GSC proliferation. c Transwell assay was employed to detect the effects of miR-103a-3p and FEZF1 on GSC migration and invasion. Representative images and accompanying statistical plots are presented. d Flow cytometry analysis of GSCs in groups according to miR-103a-3p and FEZF1(+) expression. e Effect of miR-103a-3p and FEZF1 co-transfection on the CDC25A, p-AKT and p-PI3K protein expression, with GAPDH as an endogenous control. *P < 0.05 vs. pre-NC + FEZF1 -NC group, ^#P < 0.05 vs. pre- miR-103a-3p + FEZF1-NC group. Scale bars, 20 μm. The photographs were taken at 200 × magnification. Data are presented as the mean ± SD (n = 5, each group)