Fig. 7

CDC25A was up-regulated in GSCs, and played an oncogenic role in GSCs. a Western blot analysis of CDC25A expression in non-GSCs and GSCs, with GAPDH as an endogenous control. *P < 0.05 vs. non-GSCs-U87 group, ^#P < 0.05 vs. non-GSCs-U251 group. b CCK8 assay was performed to evaluate the effect of CDC25A on the proliferation of GSCs. c Quantification of GSC migration and invasion upon CDC25A overexpression or downregulation. Representative images and accompanying statistical plots are presented. d Flow cytometry analysis of the effects of CDC25A on GSCs. e Western blot analysis of the PI3K/AKT pathway regulated by CDC25A in GSCs. Data are presented as the mean ± SD (n = 5, each group). *P < 0.05 vs. CDC25A (+)-NC group; ^#P < 0.05 vs. CDC25A (−)-NC group. Scale bars, 20 μm. The photographs were taken at 200 × magnification