Fig. 7From: The long non-coding RNA BC200 (BCYRN1) is critical for cancer cell survival and proliferationBC200 expression is reduced by confluence and serum deprivation. (a) Cells were plated at a concentration of 2 × 105 (~50% confluent) or 5 × 105 (100% confluent) and collected following 48-h culture. BC200 expression was measured by RT-qPCR and corrected to the housekeeping gene GAPDH. Data represents the mean of triplicate measurements +/− standard error. (b) Cells were plated at a concentration of 2 × 105. Following 24-h media was changed to serum free DMEM. BC200 expression was assessed 24 h following serum deprivation by RT-qPCR as in (a)Back to article page