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Fig. 5 | Molecular Cancer

Fig. 5

From: STAT3-mediated upregulation of lncRNA HOXD-AS1 as a ceRNA facilitates liver cancer metastasis by regulating SOX4

Fig. 5

HOXD-AS1 regulates SOX4 expression through a miRNA-dependent mechanism. a. RIP experiments were performed using the AGO2 antibody, and specific primers were used to detect the enrichment of HOXD-AS1 and SOX4. The data represent the average and standard deviation of three independent experiments. b Dicer deficiency rescued the siHOXD-AS1-mediated reduction of HOXD-AS1 and SOX4 mRNA in Huh7 cells. c Firefly luciferase activity normalized to Renilla luciferase activity in HEK-293 T cells co-transfected with luciferase reporters with wild type or mutant transcripts of HOXD-AS1 or SOX4 along with miR-130a-3p mimics or negative control (NC). d Protein levels of SOX4 following transfection of miR-130a-3p mimics or NC into Huh-7 and SMMC-7721 cells. e mRNA level of HOXD-AS1 was detected by biotin-coupled miR-130a-3p pull-down in Huh7 and SMMC-7721 cells. f Left: Protein level of SOX4 after transfection of miR-130a-3p mimics in SNU449 cells with HOXD-AS1 overexpression. Right: Protein level of SOX4 after transfection of miR-130a-3p inhibitors in Huh7 cells with HOXD-AS1 knockdown. Data in (b, c, e) are the means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001

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